Journal: Journal of Nanobiotechnology

Article Title: Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity

doi: 10.1186/1477-3155-9-47

Figure Lengend Snippet: Transfection efficiency

Article Snippet: The PCR products were separated on a 1.5% agarose gel, eluted using QIAquick Gel Extraction Kit (QIAGEN, Hilden, Germany), and cloned into the bicistronic pIRES-hrGFPII mammalian expression vector (Stratagene, La Jolla, CA, USA).

Techniques: Transfection

Journal: Journal of Nanobiotechnology

Article Title: Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity

doi: 10.1186/1477-3155-9-47

Figure Lengend Snippet: Transfection efficiency and toxicity of pIRES-hrGFPII- eIL-12 . GFP- (■) and PI- (◊) positive cells 24 h after transfection with pIRES-hrGFPII- eIL-12 . Mean cell proliferation (▲) (48 h and 72 h after transfection with pIRES-hrGFPII- eIL-12 ). Each bar represents a mean ± SD. * p ≤ 0.05.

Article Snippet: The PCR products were separated on a 1.5% agarose gel, eluted using QIAquick Gel Extraction Kit (QIAGEN, Hilden, Germany), and cloned into the bicistronic pIRES-hrGFPII mammalian expression vector (Stratagene, La Jolla, CA, USA).

Techniques: Transfection

Journal: Journal of Nanobiotechnology

Article Title: Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity

doi: 10.1186/1477-3155-9-47

Figure Lengend Snippet: Transfection toxicity

Article Snippet: The PCR products were separated on a 1.5% agarose gel, eluted using QIAquick Gel Extraction Kit (QIAGEN, Hilden, Germany), and cloned into the bicistronic pIRES-hrGFPII mammalian expression vector (Stratagene, La Jolla, CA, USA).

Techniques: Transfection

Journal: Journal of Nanobiotechnology

Article Title: Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity

doi: 10.1186/1477-3155-9-47

Figure Lengend Snippet: Cell proliferation after transfection

Article Snippet: The PCR products were separated on a 1.5% agarose gel, eluted using QIAquick Gel Extraction Kit (QIAGEN, Hilden, Germany), and cloned into the bicistronic pIRES-hrGFPII mammalian expression vector (Stratagene, La Jolla, CA, USA).

Techniques:

Journal: Journal of Nanobiotechnology

Article Title: Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity

doi: 10.1186/1477-3155-9-47

Figure Lengend Snippet: Immunofluorescence 24 h after transfection . pIRES-hrGFPII- eIL-12 transfection with the FHD protocol, primary antibody goat IgG anti-p35 and a donkey anti-goat secondary antibody (Texas Red fluorochrome). (a) GFP and Red Fluorescence merged image, (b) GFP Fluorescence and (c) Red Fluorescence images. Scale bar 50 μm. pIRES-hrGFP-HMGB1 transfection with the FHD protocol, primary antibody mouse anti-HMGB1 and secondary antibody goat anti-mouse (Texas Red fluorochrome). (d) GFP and Red Fluorescence merged image, (e) GFP Fluorescence and (f) Red Fluorescence images. Scale bar 75 μm.

Article Snippet: The PCR products were separated on a 1.5% agarose gel, eluted using QIAquick Gel Extraction Kit (QIAGEN, Hilden, Germany), and cloned into the bicistronic pIRES-hrGFPII mammalian expression vector (Stratagene, La Jolla, CA, USA).

Techniques: Immunofluorescence, Transfection, Fluorescence